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    D1045 5-Hydroxymethyl dCTP
    
				
      
					
    • 產品型號：
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    • 產品時間：2024-08-09
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    • 簡要描述：D1045 5-Hydroxymethyl dCTP   上?；鄯f生物優勢供應，*，！
         上?；鄯f生物，值得您信賴的選擇！！
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    D1045 5-Hydroxymethyl dCTP   
    品牌：ZYMO Research
    代理商：慧穎生物
    庫存：貨期產品
    D1045 5-Hydroxymethyl dCTP   
    Ready to use 5-Hydroxymethylcytosine mix (dATP, dTTP, dGTP, d5hmCTP) and 5-Methylcytosine dNTP mix (dATP, dTTP, dGTP, d5mCTP) is of ultra high purity; >99% trisphosphate by HPLC.
    Readily incorporated into PCR amplicons with ZymoTaq™ or other DNA polymerases.
    Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.
    About Hydroxy/Methylated Nucleotides
    5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.
     For 5-hmCFor 5-mC
    ReagentVolumeFinal ConcentrationVolumeFinal Concentration
    2X Reaction Buffer25 µl1X25 µl1X
    dNTP Mix0.5 µl0.25 mM each dNTP0.5 µl0.25 mM each dNTP
    Forward Primer (10 µM)Variable0.3 to 1 µMVariable0.3 to 1 µM
    Reverse Primer (10 µM)Variable0.3 to 1 µMVariable0.3 to 1 µM
    Template DNAVariable< 200 ng/50 µlVariable< 200 ng/50 µl
    ZymoTaq™ DNA Polymerase (5 U/µl)1 µl5 U/50 µl0.4 µl<2 U/50 µl
    ddH20to 50 µl-to 50 µl-
    Total Volume50 µl 50 µl 
     
    Note: The final concentration of MgCl2 in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl2, primer, and/or template concentrations.
    Specifications
    Concentration5-Hydroxymethylcytosine dNTP Mix: 2.5 µmol/250 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and d5hmCTP). 
    5-Hydroxymethyl dCTP: 10µmol/100 µl as a [100 mM] solution.
    5-Methylcytosine dNTP Mix: 2.5 µmol/250 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and d5mCTP).
    5-Methyl dCTP: 1µmol/100 µl as a [10 mM] solution.
    ApplicationsPCR amplicons containing 5-(hydroxy)methylcytosine are useful in a variety of applications such as: 
    • The study of 5-(hydroxy)methylcytosine binding/interacting protein
    • Define methods that distinguish between 5-methylcytosine and 5-hydroxymethylcytosine.
    Storage5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP are guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.
    
			
    
		
    
		
		

		
		
    
			



    

    
	
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